S. Ulianov, E. Khrameeva, A. Gavrilov, I. Flyamer, P. Kos, E. Mikhaleva, A. Penin, M. Logacheva, M. Imakaev, A. Chertovich, M. Gelfand, Y. Shevelyov, and S. Razin
Recent advances enabled by the Hi-C technique have unraveled many principles of chromosomal folding that were subsequently linked to disease and gene regulation. In particular, Hi-C revealed that chromosomes of animals are organized into Topologically Associating Domains (TADs), evolutionary conserved compact chromatin domains that influence gene expression. Mechanisms that underlie partitioning of the genome into TADs remain poorly understood. To explore principles of TAD folding in Drosophila melanogaster, we performed Hi-C and PolyA+ RNA-seq in four cell lines of various origins (S2, Kc167, DmBG3-c2, and OSC).